An accurate and streamlined approach to differential display (DD) band identification and verification is described.To minimize false positives, the strategy avoids the use of impure Northern blot probes obtained from PCR-amplified DD bands.To increase throughput, the cloning of DD bands is replaced by a gene-specific primer approach, and hybridiza
The 80% ethanol extract of Alstonia yunnanensis afforded five new monoterpenoid indole alkaloids: 11-hydroxy-6,7-epoxy-8-oxo-vincadifformine (1), 14-chloro-15-hydroxy- vincadifformine (2), perakine N4-oxide (3), raucaffrinoline N4-oxide (4), and vinorine N1,N4-dioxide (5), together with three known compounds: 11-methoxy-6,7-epoxy-8-oxo- vincadiffor
Abstract Water uptake is the fundamental requirement for the initiation and completion of seed germination that is a vital phase in the life cycle of seed plants.We found that seeds produced under four nitrogen levels showed significantly different germination speed.The scandinavian edition flair objective of this study was to study the mechanism o
In recent years, the demand for nutritive, functional and healthy foods has increased.This trend has induced the food industry to investigate novel technologies able to produce ingredients with enhanced functional and physicochemical properties.Among these technologies, one of the most promising is the encapsulation based on supercritical fluids.Th
Medical management and lifestyle changes rather than surgical interventions, are used for many urological problems.This was also the case in the past.The use of herbal medication for urinary symptoms in the nineteenth century is reviewed as well as the acceptance of herbal scandinavian edition flair medicine at that time.The place of thia-cal horse